1.所有问题必须逐条回答。
2.尽量满足意见中需要补充的实验。
3.满足不了的也不要回避，说明不能做的合理理由。
4.审稿人推荐的文献一定要引用，并讨论透彻。
以下是本人对审稿人意见的回复一例，仅供参考。
Major comments:
1. The authors need to strengthen their results by including MMP
secretion, and tran-matrigel migration by a positive control
progenitor cell population i.e. enriched human CD34 cells
obtained from mobilized PBL, since this is a more clinically
relevant source of CD34 cells which has also been shown to
secrete both MMP-9 and MMP-2 (ref. 11). CD34 enriched cells
from steady state peripheral blood which also secrete MMPs are
also of interest.
2. In fig 1C please specify which cell line represents
MMP-negative cells. This needs to be clarified, as well as a
better explanation of the method of the protocol.
3. The ELISA results are represented as "fold increase" compared
to control. Instead, we suggest that standards should be used and
results should be presented as absolute concentrations and only
then can these results be compared to those of the zymography.
4. When discussing the results, the authors should distinguish
clearly between spontaneous migration vs chemotactic migration.
Furthermore, the high spontaneous migration obtained with cord
blood CD34 cells should be compared to mobilized PBL CD34
enriched cells and discussed.
5. The authors claim that the clonogenic assay was performed to
determine the optimum concentration for inhibition of MMP
activity by phenanthroline and anti MMP-9 mAb, however they
should clarify that this assay can only determine the toxicity of
the inhibitors and not their optimal inhibitory concentrations.
Minor comments:
1. There are many spelling and syntax errors, especially in the
results and discussion, which need correction.
a. Of special importance, is the percent inhibition of migration,
which is described as percent of migration. i.e. pg 7:"Migration
of CB CD34 was reduced to 73.3%?" Instead should read
"Migration of CB CD34 was reduced by 73.3%?"
b. The degree symbol needs to be added to the numbers in
Materials and methods.
2. It would be preferable to combine figure 1A and B, in order to
confirm the reliability of fig. 1B by a positive control
(HT1080).
